CULTIVO DE STEVIA EN COLOMBIA EPUB DOWNLOAD

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CULTIVO DE STEVIA EN COLOMBIA EPUB DOWNLOAD

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Stevia rebaudiana Bertoni es una plantaque produce en las hojas un Japón, Brasil y China, y últimamente ha despertado gran interés en Colombia. como pueden ver tengo semilla de estevia dentro de unfrasco de cafe que he mantenido refrigerada. hay que. Las hojas de Stevia rebaudiana y sus glucósidos re- cientemente se han . sayo se realizó en placas de cultivo celular de 96 pozos. y a cada pozo se le.


CULTIVO DE STEVIA EN COLOMBIA EPUB DOWNLOAD

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Stevia: The Genus Stevia - Google Livros

The highest mean number of embryos was achieved in the treatments containing the highest concentration of 2iP 7. The media containing Zeatin showed the formation of somatic embryos, but they were deformed and had irregular morphological characteristics.

Response of leaf segments from in vitro and ex vitro plants to the formation of somatic embryos This assay revealed that the origin of the explant had an effect on the response time but not on the total number of somatic embryos. The leaf segments from ex vitro plants began forming somatic embryos cultivo de stevia en colombia 25 and 27 days after planting, whereas the segments from in vitro plants responded after 20 days.

The statistical analysis performed with the data from 40 days after planting showed significant differences in the number of embryos obtained in the leaf explants from these two origins Fig.

However, when this same parameter was evaluated at the 60th day, no statistically significant differences were observed Fig. Results 40 days after planting; b.

However, similar to genotype 2, a lower response time was observed in the material from in vitro conditions compared with the ex vitro plants under semi-control conditions at 30 and 45 days, respectively.

Effect of the genotype on the induction of somatic embryos The response to embryogenesis in all three S. Genotype 2 SRQ achieved a mean of 30 embryos per explant.

CULTIVO DE STEVIA EN COLOMBIA EPUB DOWNLOAD

In genotype 1 Bertonithe response was the formation of an embryonic callus, and only one explant achieved formation of a somatic embryo. For genotype 3 Morita IIthe response was the formation of a compact dark yellow-colored cultivo de stevia en colombia with no embryogenic characteristics Fig.

Figure 3 Effect of genotype on the induction of somatic embryos in S.

Cultivation Generalities

All of these characteristics are typical of meristematic cells with regeneration potential; in cultivo de stevia en colombia case, the potential is through somatic embryogenesis Fig.

Similarly, the tissues surrounding the embryos were observed to correspond to the mother tissue and were characterized by parenchymal cells Fig. Regeneration of somatic embryos The conversion of the somatic embryo to a plant was only obtained in the R1 media and achieved days after transfer to the regeneration media Fig.

In the R2 media, the embryos remained intact after days with no regeneration. However, when the embryos were transferred to the R1 medium, seedlings were obtained after 20 days. In R3, R4 and R5 media, the embryos were covered by calli after 20 to 25 days after transfer without achieving any conversion.

Figure 4 Somatic embryos of S. Coconut water, a supplement for cultivo de stevia en colombia vitro culture, has been reported as beneficial for induction processes such as morphogenesis, somatic embryogenesis, callus formation, and for cell cultures in suspension Pervin et al.

In embryogenic processes, the production of somatic embryos at a large scale was improved in Phoenix dactylifera Hussam and Hussein, In addition, the promotion of the induction, growth and development of somatic embryos in other species was achieved Bhattacharya et al. Additionally, the adenine negatively affected the embryogenesis process in S.

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In other species, reports have noted the favorable effects of adenine on the efficiency of the induction and further development of somatic embryos Jha et al. This vitamin has been used as a supplement in culture media for cultivo de stevia en colombia induction of somatic embryos Milojevic et al.